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Target gene expression levels were normalized by 18 S ribosomal RNA (18 S ribosomal N1), and relative gene expression was calculated using the 2e(-Ct) method 21

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ROS are unstable derivatives of oxygen and can seriously damage living cells if formed in significant amounts (oxidative stress)

Ethics statement The trial was conducted in accordance with the Declaration of Helsinki (1996 revision) and under the principals of good clinical practice, as laid out in the International Conference on Harmonization document Good Clinical Practice Consolidated Guideline

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Autoclave: Autoclave Autoclave is important equipment, used to preserve the sterility of sterile preparations